Masoumeh Ebtekar, vice president of Iran for Women and Family Affairs, has tested positive for the coronavirus.
The vice president is also known as the English-language spokeswoman for the 1979 hostage-takers who seized the US embassy in Tehran and sparked the 444-day diplomatic crisis.
On Tuesday, Iranian officials confirmed that Deputy Health Minister Iraj Harirchi had been infected and was under quarantine.
Coronavirus is spreading to more countries around the world, with Estonia, Denmark and Georgia confirming first cases.The coronavirus is spreading more quickly in Europe, the Middle East and other parts of the world than in China where the virus first emerged in the central city of Wuhan at the end of last year.
The number of new infections inside China was for the first time overtaken by fresh cases elsewhere on Wednesday, with Italy, Iran and South Korea emerging as new hotspots for COVID-19.
The disease was also detected for the first time in Estonia, Denmark, Georgia, Brazil, Pakistan, Sweden, Norway, Greece, Romania and Algeria
